Background
Atherosclerotic cardiovascular diseases are the most common cause of mortality. So far, interventional management of atherosclerosis has been physical like angioplasty, atherectomy and stenting. Physical interventions rarely remove the plaque, have significant limitations and complications with possibility of restenosis and require long-term use of antiplatelets with risk of bleeding and death. In this study, we tested the hypothesis that chemicals can dissolve plaque effectively in atherosclerotic rabbits’ aortas.
Methods
Sixteen segments of atherosclerotic rabbits’ aortas were soaked in about 15 ml of different solvents (propylene glycol 99.9%, glycerine 100%, d -limonene 100%, methanol, ethanol, octanoic acid 98%, 2-octanone 98%, diethyl ether 99.7%) for 10–16 min. The treated atherosclerotic segments were compared to four untreated control atherosclerotic segments using gross morphology, hematoxylin and eosin stain (10 segments) and frozen sections (10 segments) with special fat staining (Sudan black and oil red O). Evaluation of solvents’ effect was performed by an experienced pathologist and expressed as percentage of lipid removal determined by examination of digital images in combination with examination of the slides with the various staining procedures.
Methods
Sixteen segments of atherosclerotic rabbits’ aortas were soaked in about 15 ml of different solvents (propylene glycol 99.9%, glycerine 100%, d -limonene 100%, methanol, ethanol, octanoic acid 98%, 2-octanone 98%, diethyl ether 99.7%) for 10–16 min. The treated atherosclerotic segments were compared to four untreated control atherosclerotic segments using gross morphology, hematoxylin and eosin stain (10 segments) and frozen sections (10 segments) with special fat staining (Sudan black and oil red O). Evaluation of solvents’ effect was performed by an experienced pathologist and expressed as percentage of lipid removal determined by examination of digital images in combination with examination of the slides with the various staining procedures.
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