Understanding the impact of drug-eluting stent on vascular function and the nitric oxide pathway genes using a pig coronary artery stent implantation model




Objective


Drug-eluting stents (DES) are known to impact vascular functions. The impact of the polymers and the drugs used in DES on the pathways regulating vascular reactivity is not well understood. The objective of the present study was to investigate the effect of rapamycin class of DES on vascular reactivity and the genes involved in the vasoregulating nitric oxide pathway.




Methods


A porcine model of stent implantation was used. Bare-metal stents (BMS), DES, sirolimus, zotarolimus or other limus stents were placed in two epicardial vessels of each animal with S/A ratio of 1.1:1 and overlapping segment of 1/3 to 1/2 of single stent length. After 28 days, vascular function was determined by acetylcholine (Ach) challenge. Tissue samples from the stented segment of four arteries from each group were collected. RNA and proteins were isolated. Selected genes and proteins regulating the nitric oxide pathway were further analyzed by real-time polymerase chain reaction (PCR) and western blotting.

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Nov 16, 2017 | Posted by in CARDIOLOGY | Comments Off on Understanding the impact of drug-eluting stent on vascular function and the nitric oxide pathway genes using a pig coronary artery stent implantation model

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