Background

Previous studies have described potential antiatherogenic effects of everolimus after systemic and local stent delivery. However, direct effects of everolimus on foam cells (FC) have not been well characterized. We used an in vitro model to explore the effects of everolimus on macrophage-derived FC.

Methods

FC were derived from human THP1 macrophages via incubation with acetylated LDL (100 μg/ml) for 72 h, followed by everolimus treatment (10 ⁻⁵ –10 ⁻¹¹ M) for 24 h ( n =4). FC viability was quantified using calcein AM/DAPI staining via fluorescent microscopy. FC lysates and media supernatants were analyzed for apoptosis/necrosis using a Cell Death Detection ELISA ^PLUS assay (Roche, Pleasanton, CA, USA). Media supernatants were also analyzed for inflammatory cytokines (IL1β, IL8, MCP1, TNFα) using a Procarta immunoassay (Affymetrix, Santa Clara, CA, USA). Gene expression of autophagy (MAP1LC3A), apoptosis (survivin, clusterin), and matrix degradation (MMP1, MMP9) markers were evaluated by a Quantigene Plex assay (Affymetrix). Statistical significance was calculated using one-way ANOVA ( P <.05).

Only gold members can continue reading. Log In or Register to continue

Share this:

• Click to share on Twitter (Opens in new window)
• Click to share on Facebook (Opens in new window)

Related

Related posts:

1. Left main coronary stenting in a non surgical octogenarian population: a possible approach
2. Shorter degradation period in BTI stents after porcine coronary artery implantation
3. The true incidence of atrial fibrillation after an atrial flutter ablation with continuous monitoring
4. Apical motion abnormality in patients undergoing transapical aortic valve replacement is frequent, yet spontaneously reversible

Stay updated, free articles. Join our Telegram channel

Join

Full access? Get Clinical Tree