Background
High-cholesterol diet induces atherosclerotic lesions which can be accelerated by denudation of endothelium (EC). EC denudation of iliac artery (IA) has not been well studied in the hyperlipemic rabbit. In this study, endothelial function and development atherosclerosis were investigated in nonstented (NSIA) and/or stented iliac artery (SIA).
Methods
Nine rabbits were fed with atherogenic diet for 9 weeks. Cholesterol was measured before and after 1 week, 5 weeks, and 9 weeks of diet. At 1 week, 8 IAs were denuded EC (Group II) and 10 IAs kept the EC intact (Group I). At 5 weeks, eight BMSs (3.0/15 mm) were, respectively, implanted in Group Ib and Group IIb. At 9 weeks, the animals were sacrificed followed by angiograph, OCT, and IVUS (VH) images. NSIAs (Group Ia and IIa) were examined using organ chamber for vascular function. SIAs were embedded in plastic and stained with H&E and Movat. Aorta and NSIAs were embedded in paraffin and stained with H&E, Movat, SMA, and RAM-11.
Methods
Nine rabbits were fed with atherogenic diet for 9 weeks. Cholesterol was measured before and after 1 week, 5 weeks, and 9 weeks of diet. At 1 week, 8 IAs were denuded EC (Group II) and 10 IAs kept the EC intact (Group I). At 5 weeks, eight BMSs (3.0/15 mm) were, respectively, implanted in Group Ib and Group IIb. At 9 weeks, the animals were sacrificed followed by angiograph, OCT, and IVUS (VH) images. NSIAs (Group Ia and IIa) were examined using organ chamber for vascular function. SIAs were embedded in plastic and stained with H&E and Movat. Aorta and NSIAs were embedded in paraffin and stained with H&E, Movat, SMA, and RAM-11.
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