Rapid On-Site Cytology Evaluation



Fig. 11.1
Diff-Quik® staining kit




  • A bottle of tap water


  • Bottles with fixing alcohol and staining solutions


  • Dryer


  • Microscope






       

    • 2.


      Rapid Papanicolaou stain (Fig. 11.2):

      A340824_1_En_11_Fig2_HTML.jpg


      Fig. 11.2
      A portion of the rapid Papanicolaou staining set




      • Ninety-five percent ethanol


      • One-hundred percent ethanol


      • Hydrochloric acid


      • Gill’s hematoxylin


      • OG-6


      • EA50


      • Xylene


      • Mounting medium


      • Cover glasses


      • Microscope

       






        11.3 Procedure for ROSE





        1. 1.


          Diff-Quik® stain (Fig. 11.3):

          A340824_1_En_11_Fig3_HTML.jpg


          Fig. 11.3
          Slide preparation stained by Diff-Quik®



          1. (a)


            Use cold air to dry the glass slides that contain the brush smears or imprints of specimens.

             

          2. (b)


            Dip the sample on the glass slide in the fixing alcohol of the staining kit for 10 s.

             

          3. (c)


            Stain for 20 s in solution I of the staining kit.

             

          4. (d)


            Stain for 20 s in solution II of the staining kit.

             

          5. (e)


            Rinse the stained slide preparation with water, and apply hot air to dry.

             

          6. (f)


            Observe by microscopy.

             

           

        2. 2.


          Rapid Papanicolaou stain (Fig. 11.4):

          A340824_1_En_11_Fig4_HTML.jpg


          Fig. 11.4
          Slide preparation stained by rapid Papanicolaou



          1. (a)

        1. Tags:
        Sep 25, 2017 | Posted by in RESPIRATORY | Comments Off on Rapid On-Site Cytology Evaluation

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