Processing Samples



Fig. 10.1
Preparation of transbronchial biopsy/cytology samples






  1. 1.


    Specimen bottles with formalin

     

  2. 2.


    Thin tweezers

     

  3. 3.


    Nitrocellulose papers with square cut sections that are 5-mm wide

     

  4. 4.


    Glass slides

     

  5. 5.


    Glass slide bottles with fixing alcohol

     

  6. 6.


    Plastic tubes with small amount (about 2 ml) of saline

     

  7. 7.


    Several pieces of gauze

     



10.3 The Actual Procedure





  1. 1.


    Preparation of transbronchial biopsy specimens

    Using thin tweezers, transfer specimens obtained by forceps on nitrocellulose paper (Fig. 10.2) before placing in a formalin bottle (Fig. 10.3).

    A340824_1_En_10_Fig2_HTML.jpg



    Fig. 10.2


    A340824_1_En_10_Fig3_HTML.jpg



    Fig. 10.3

     

  2. 2.


    Handling of the brush samples:


    1. (a)


      Smear the tip of the brush on a pair of glass slides (Fig. 10.4).

      A340824_1_En_10_Fig4_HTML.jpg



      Fig. 10.4

       

    2. (b)


      Put one glass slide in a bottle containing the fixing alcohol (Fig. 10.5).

      A340824_1_En_10_Fig5_HTML.jpg



      Fig. 10.5

       

    3. (c)


      The other glass slide is air-dried for rapid on-site examination (Fig. 10.6). If rapid on-site cytology is not necessary, no additional glass slide is needed.
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Sep 25, 2017 | Posted by in RESPIRATORY | Comments Off on Processing Samples

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