1
Principles of Echocardiographic Image Acquisition and Doppler Analysis
Ultrasound Waves
Sound waves are mechanical vibrations that induce alternate refraction and compression of any physical medium through which they pass (Fig. 1-1). Like other waves, sound waves are described in terms of (Table 1-1):
Frequency (f) is the number of ultrasound waves in a 1-second interval. The units of measurement are hertz, abbreviated Hz, which simply means cycles per second. A frequency of 1000 cycles/s is 1 kilohertz (KHz), and 1 million cycles/s is 1 megahertz (MHz). Humans can hear sound waves with frequencies between 20 Hz and 20 kHz; frequencies higher than this range are termed ultrasound. Diagnostic medical ultrasound typically uses transducers with a frequency between 1 and 20 MHz.
Since the propagation velocity in the heart is constant at 1540 m/s, the wavelength for any transducer frequency can be calculated (Fig. 1-2) as:
Figure 1–2 Transducer frequency versus wavelength and penetration of the ultrasound signal in soft tissue.
Wavelength has been plotted inversely to show that resolution increases with increasing transducer frequency while penetration decreases. The specific wavelengths for transducer frequencies of 1, 2.5, 3.5, 5, and 7.5 MHz are shown.
For example, the wavelength emitted by a 5 MHz transducer can be calculated as:
Wavelength is important in diagnostic applications for at least two reasons:
Image resolution is no greater than 1 to 2 wavelengths (typically about 1 mm).
The depth of penetration of the ultrasound wave into the body is directly related to wavelength; shorter wavelengths penetrate a shorter distance than longer wavelengths.
Thus, a ratio of 1000 to 1 is:
A simple rule to remember is that a 6-dB change represents a doubling or halving of the signal amplitude or that a 40-dB change represents a 100 times difference in amplitude (Fig. 1-3). If acoustic intensity is used instead of amplitude, the constant 10 replaces 20 in the equation so that a 3 dB changes represents doubling, and a 20 dB change indicates a 100-fold difference in amplitude. Either of these decibel scales may be used to refer to transmitted or received ultrasound waves or to describe attenuation effects. The advantages of the decibel scale are that a very large range can be compressed into a smaller number of values, and that low-amplitude (weak) signals can be displayed alongside very high-amplitude (strong) signals. In an echocardiographic image, amplitudes typically range from 1 to 120 dB. The decibel scale is the standard format both for echocardiographic image display and for the Doppler spectral display, although other amplitude scales may be an option.
Figure 1–3 Graph of the decibel scale.
The logarithmic relationship between the decibel scale (horizontal axis) and the amplitude ratio (vertical axis) is seen. A doubling or halving of the amplitude ratio corresponds to a 6-dB change, and a 100-fold difference in amplitude corresponds to a 20-dB change.
Ultrasound Tissue Interaction
Propagation of ultrasound waves in the body to generate ultrasound images and Doppler data depends on a tissue property called acoustic impedance (Table 1-2). Acoustic impedance (Z) depends on tissue density (ρ) and on the propagation velocity in that tissue (c):
The interaction of ultrasound waves with the organs and tissues of the body can be described in terms of (Fig. 1-4):
Figure 1–4 Diagram of the interaction between ultrasound and body tissues.
Doppler analysis is based on the scattering of ultrasound in all directions from moving blood cells with a resulting change in frequency of the ultrasound received at the transducer. 2D imaging is based on reflection of ultrasound from tissue interfaces (specular reflectors). Attenuation limits the depth of ultrasound penetration. Refraction, a change in direction of the ultrasound wave, results in imaging artifacts.
Scattering
The extent of scattering depends on:
Scattering also occurs within tissues, such as the myocardium, from interference of backscattered signals from tissue interfaces smaller than the ultrasound wavelength. Tissue scattering results in a pattern of speckles; tissue motion can be measured by tracking these speckles from frame to frame, as discussed in Chapter 4.
Attenuation
The attenuation coefficient (α) for each tissue is related the decrease in ultrasound intensity (measured in dB) from one point (I1) to a second point (I2) separated by a distance (l) as described by the equation:
The power output of the transducer is directly related to the overall degree of attenuation. However, an increase in power output may cause thermal and mechanical bioeffects as discussed in Bioeffects and Safety, p. 27.
Transducers
Ultrasound transducers use a piezoelectric crystal both to generate and to receive ultrasound waves (Fig. 1-5). A piezoelectric crystal is a material (such as quartz or a titanate ceramic) with the property that an applied electric current results in alignment of polarized particles perpendicular to the face of the crystal with consequent expansion of crystal size. When an alternating electric current is applied, the crystal alternately compresses and expands, generating an ultrasound wave. The frequency that a transducer emits depends on the nature and thickness of the piezoelectric material.
Figure 1–5 Schematic diagram of an ultrasound transducer.
The piezoelectric crystal both produces and receives ultrasound signals, with the electric input-output transmitted to the instrument via the cable. Damping material allows a short pulse length (improved resolution). The shape of the piezoelectric crystal, an acoustic lens, or electronic focusing (with a phased-array transducer) are used to modify beam geometry. The material of the transducer surface provides impedance matching with the skin. The ultrasound pulse length for 2D imaging is short (1-6ms), typically consisting of two wavelengths (λ). “Ring down”—the decrease in frequency and amplitude in the pulse—depends on damping and determines bandwidth (the range of frequencies in the signal).
The burst, or pulse, of ultrasound generated by the piezoelectric crystal is very brief, typically 1 to 6 µs, because a short pulse length results in improved axial (along the length of the beam) resolution. Damping material is used to control the ring-down time of the crystal and, hence, the pulse length. Pulse length also is determined by frequency because a shorter time is needed for the same number of cycles at higher frequencies. The number of ultrasound pulses per second is called the pulse repetition frequency, or PRF. The total time interval from pulse to pulse is called the cycle length, with the percent of the cycle length used for ultrasound transmission called the duty factor. Ultrasound imaging has a duty factor of about 1% compared to 5% for pulsed Doppler and 100% for continuous-wave (CW) Doppler. The duty factor is a key element in the patient’s total ultrasound exposure as discussed in Bioeffects and Safety, p. 27.
Types of Transducers
The simplest type of ultrasound transducer is based on a single piezoelectric crystal (Table 1-3). Alternate pulsed transmission and reception periods allow repeated sampling along a single line, with the sampling rate limited only by the time delay needed for return of the reflected ultrasound wave from the depth of interest. An example of using the transducer for simple transmission-reception along a single line is an A-mode (amplitude versus depth) or M-mode (depth versus time) cardiac recording when a high sampling rate is desirable.
Formation of more complex images uses an array of ultrasound crystals arranged to provide a 2D tomographic or 3D volumetric data set of signals. Each element in the transducer array can be controlled electronically both to direct the ultrasound beam across the region of interest and to focus the transmitted and received signals. Echocardiographic imaging uses a sector scanning format with the ultrasound signal originating from a single location (the narrow end of the sector), resulting in a fanlike shape of the image. Sector scanning is optimal for cardiac applications because it allows a fast frame rate to show cardiac motion and a small transducer size (aperture or “footprint”) to fit into the narrow acoustic windows used for echocardiography. Three-dimensional imaging transducers are discussed in Chapter 4.
Beam Shape and Focusing
An unfocused ultrasound beam is shaped like the light from a flashlight, with a tubular beam for a short distance that then diverges into a broad cone of light (Fig. 1-6). Even with current focused transducers, ultrasound beams have a 3D shape that affects measurement accuracy and contributes to imaging artifacts. Beam shape and size depend on several factors, including:
Figure 1–6 Schematic diagram of beam geometry for an unfocused (left) and focused (right) transducer.
The length of the near zone and the divergence angle in the far field depend on transducer frequency and aperture. The focal zone of a focused transducer can be adjusted, but beam width still depends on depth. Side lobes (and grating lobes with phased-array transducers) occur with both focused and unfocused transducers and, like the central beam, are 3D.
This equation indicates a divergence angle of 6° beyond the near field, resulting in an ultrasound beam width of about 4.4 cm at a depth of 20 cm for this 3.5-MHz transducer. With a 10-mm diameter aperture, Fn would be 5.7 cm and beam width at 20 cm would be about 2.5 cm (Fig. 1-7).
Figure 1–7 Transducer frequency versus near zone length and divergence angle.
Transducer frequency is shown on the horizontal axis with the length of the near zone shown in yellow and divergence angle in blue for unfocused 5- (squares) and 10-mm (triangles) diameter aperture transducers. Equations (1-6) and (1-7) were used to generate these curves.
In addition to the main ultrasound beam, dispersion of ultrasound energy laterally from a single-crystal transducer results in formation of side lobes at an angle θ from the central beam where sin θ = m λ/D, and m is an integer describing sequential side lobes (i.e., 1, 2, 3, and so on) (Fig. 1-8). Reflected or backscattered signals from these side lobes may be received by the transducer, resulting in image or flow artifacts. With phased-array transducers, additional accessory beams at an even greater angle from the primary beam, termed grating lobes, also occur as a result of constructive interference of ultrasound wave fronts. Both the side lobes and the grating lobes affect the lateral and elevational resolution of the transducer.
Figure 1–8 Transducer beam side lobes.
Top: This diagram shows that side lobes occur at the points where the distances traversed by the ultrasound pulse from each edge of the crystal face differ by exactly one wavelength. The distance from the left edge of the crystal (P1) to the position of side lobe 1 is exactly one wavelength (λ) longer than the distance from the extreme right edge of the crystal (P2) to the position of side lobe 1. Bottom: The beam intensity plot formed by sweeping along an arc at focal length F. (From Geiser EA: Echocardiography: physics and instrumentation. In Skorton DJ, Schelbert AR, Wolf GL, Brundage BH [eds]: Marcus Cardiac Imaging, 2nd ed. Philadelphia: WB Saunders, 1996, p 280. Used with permission.)
Resolution
Image resolution occurs for each of three dimensions (Fig. 1-9):
Figure 1–9 Axial, lateral, and elevational slice thickness in three dimensions for a phased-array transducer ultrasound beam.
A, Axial resolution along the direction of the beam is independent of depth; lateral resolution and elevational resolution are strongly depth dependent. Lateral resolution is determined by transmit and receive focus electronics; elevational resolution is determined by the height of the transducer elements. At the focal distance, axial is better than lateral and is better than elevational resolution. B, Elevational resolution profile with an acoustic lens across the transducer array produces a focal zone in the slice thickness direction. (From Bushberg JT, et al: The Essential Physics of Medical Imaging. Philadelphia: Lippincott Williams & Wilkins, 2002, Fig. 16-21).
Axial resolution along the length of the ultrasound beam
Lateral resolution side to side across the 2D image
Elevational resolution or thickness of the tomographic slice
Of these three, axial resolution is most precise, so quantitative measurements are made most reliably using data derived from a perpendicular alignment between the ultrasound beam and structure of interest. Axial resolution depends on the transducer frequency, bandwidth, and pulse length but is independent of depth (Table 1-4). Determination of the smallest resolvable distance between two specular reflectors with ultrasound is complex but is typically about twice the transmitted wavelength; higher-frequency (shorter-wavelength) transducers have greater axial resolution. For example, with a 3.5 MHz transducer, axial resolution is about 1 mm, versus 0.5 mm with a 7.5 MHz transducer. A wider bandwidth also improves resolution by allowing a shorter pulse, thus avoiding overlap between the reflected ultrasound signals from two adjacent reflectors.
Table 1-4
Determinants of Resolution in Ultrasound Imaging
Axial Resolution
Transducer frequency
Transducer bandwidth
Pulse length
Lateral Resolution
Transducer frequency
Beam width (focusing) at each depth∗
Aperture (width) of transducer
Bandwidth
Side and grating lobe levels
Elevational Resolution
Transducer frequency
Beam width in elevational plane
Lateral resolution varies with the depth of the specular reflector from the transducer, primarily related to beam width at each depth. In the focal region where beam width is narrow, lateral resolution may approach axial resolution, and a point target will appear as a point on the 2D image. At greater depths, beam width diverges so a point target results in a reflected signal as wide as the width of the beam, which accounts for “blurring” of images in the far field. If the 2D image is examined carefully, progressive widening of the echo signals from similar targets along the length of the ultrasound beam can be appreciated (Fig. 1-10). Erroneous interpretations occur when the effects of beam width are not recognized. For example, beam width artifact from a strong specular reflector may appear to be an abnormal linear structure. Other factors that affect lateral resolution are transducer frequency, aperture, bandwidth, and side and grating lobe levels.