Objectives

Acute mesenteric ischemia (AMI) is a rapidly progressive disease where early diagnosis is life-saving. In our study, we obtained changes of visfatin levels in the serum, peritoneal and intestinal lavage samples in rats, to investigate the effectiveness of these changes in the early diagnosis of AMI.

Material-Methods

In Group 1 (Sham group) the intestine was pulled out and allowed to stand for 3 hours without ischemia. In group 2 (acute mesenteric ischemiareperfusion group) the mesenteric artery was ligated and, mesenteric blood flow was restored after 60 min ischemia. In Group 3 (acute pancreatitis group) the ductus pancreaticus was ligated, and the abdomen was closed for 3 days in expectation of the formation of pancreatitis. In all of the groups, the intestinal lavage, peritoneal lavage and blood samples were analyzed in 0, 60 and 180 minutes.

Material-Methods

In Group 1 (Sham group) the intestine was pulled out and allowed to stand for 3 hours without ischemia. In group 2 (acute mesenteric ischemiareperfusion group) the mesenteric artery was ligated and, mesenteric blood flow was restored after 60 min ischemia. In Group 3 (acute pancreatitis group) the ductus pancreaticus was ligated, and the abdomen was closed for 3 days in expectation of the formation of pancreatitis. In all of the groups, the intestinal lavage, peritoneal lavage and blood samples were analyzed in 0, 60 and 180 minutes.

Results

Serum, intestinal and peritoneal lavage visfatin levels were found to be increased in Group 2 and Group 3 (P <0.05). In Group 2, while serum TNF-alpha levels were increased in both ischemia and reperfusion. In intestinal lavage sample the increase was only in the ischemic phase. Serum and intestinal TNF-alfa levels were increased in Group 3 (p <0.05). IL-8 seviyeleri ise; Grup 2’de serumda iskemi sonrası (p=0,03) ve intestinal lavajda reperfüzyon sonrası anlamlı artış (p=0,004) göstermiştir. Grup 3’de ise sadece peritoneal lavaj örneklerinde anlamlı artış saptanmıştır (p=0,049).

In Group 2, IL-8 levels were significantly increased after ischemia in serum (p = 0.03) and after reperfusion in intestinal lavage (p = 0.004) samples. In Group 3, the only significant increase was observed in the in peritoneal lavage samples (p = 0.049).