Objective
Sirtuin1 (SIRT1) is a member of the Sirtuin family of class III histone deacetylases (HDACs) and a longevity factor in mammals initiating the cell survival mechanisms, as well as preventing the ischemic injury in heart. In Heart Failure (HF), an impairment in cell survival mechanism of cardiomyocytes is a notable factor in disease etiopathogenesis. Among many underlining factors, it is well known that oxidative stress comprises a critical impact on cardiomyocyte survival in HF. To date, less scrutiny has been given to the role of SIRT1 in patients with HF. The aim of the present study was to investigate expression levels of SIRT1 gene in compensated and decompensated HF groups, and their correlation with oxidative stress.
Methods
Study populations of 156 patients (80 compensated and 76 decompensated) and 80 healthy volunteers were included in the study. The expression levels of SIRT1 in human peripheral blood mononuclear cells from patients with HF were examined using quantitative real-time polymerase chain reaction (qRT-PCR). Oxidative stress parameters including the levels of total oxidant status (TOS; in μmol H2O2 equivalents /l), total antioxidant status (TAS; in μmol Trolox Eq/L) were measured using commercially available kits via colorimetric assays in blood serum samples, and oxidative stress index (OSI) was calculated. Lipid parameters were determined by routine laboratory methods.
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