Objectives
SH are sulfur analogs of alcohols and founds as frees and oxidized form in plasma. The plasma SH source is mainly consisting for the glycine, glutamic acid, cysteine tripeptide and glutathione. It constitutes of the cells important antioxidant status. The balance of oxidant and antioxidant status plays an important role formation of LVDD in patients with hypertension. We hypothesized that SH levels may be associated with LVDD in those patients. The aim of the study was investigated the relationship between plasma thiol (SH) levels and left ventricular diastolic dysfunction (LVDD) in patient with hypertension.
Methods
A total of 158 subjects, 138 of them with hypertensive and ejection fraction (EF) >50% patients, 20 age-gender matched controls. Hypertensive patients were divided three groups: Group I; the subjects without LVDD (n=41), Group II; the patients with relaxation abnormalities of LVDD (n=57), Group III; the patients with pseudonormalization pattern (n=40). Plasma SH levels were measured by a novel and automated spectrophotometric method.
Methods
A total of 158 subjects, 138 of them with hypertensive and ejection fraction (EF) >50% patients, 20 age-gender matched controls. Hypertensive patients were divided three groups: Group I; the subjects without LVDD (n=41), Group II; the patients with relaxation abnormalities of LVDD (n=57), Group III; the patients with pseudonormalization pattern (n=40). Plasma SH levels were measured by a novel and automated spectrophotometric method.
Results
All population’s finding of clinical, echocardiographic, and biochemical are shown in table-1. Age, systolic and diastolic pressures, EF, using diastolic dysfunction parameters in echocardiography and plasma SH levels are significantly different among the groups. The lowest SH level are found in group III, and highest levels in control (Figure-1). Univariate analysis indicated that the presence of LVDD was correlated with age, SH levels, history of coronary artery diseases and EF (all of p<0.05). But, age and SH were independently predictors of LVDD in multivariate analyses (ß=-0.318, p<0.001, and ß=0.314, p<0.001, respectively). ROC- curve analysis revealed that thiol levels over 163 μmol/L predicted LVDD in hypertensive patients with 75% sensitivity and 70% specificity (area under the curve=0.783; 95% CI 0.714-0.852).
Conclusions
Plasma SH is an independent predictor for the presence of LVDD. This finding supports a role for SH in the pathogenesis of diastolic function, and its increased of the levels may help prevent for developing diastolic dysfunction.
