Methods
In Wistar albino rats, cathecolamine induced cardiac damage was induced by intraperitoneal ISO (5 mg/kg) injections for 30 days. During this period, half of the rats were treated with saline while the other half were given RVT (10 mg/kg/day). At the end of this period, blood pressure and echocardiographic measurements were recorded and the rats were decapitated to obtain plasma and cardiac tissue samples. Lactate dehydrogenase (LDH), creatine kinase (CK), lysosomal enzymes (β-D-glucuronidase, β-galactosidase, β-D-N-acetyl-glucosaminidase, acid phosphatase and cathepsin-D) were studied in plasma samples, while malondialdehyde (MDA), ATP and glutathione (GSH) levels and Na+/K+ ATPase and myeloperoxidase (MPO) activities were determined in the cardiac tissue samples. Caveolin-3 levels in cardiac tissues were evaluated using both histological and Western blot analyses.
Methods
In Wistar albino rats, cathecolamine induced cardiac damage was induced by intraperitoneal ISO (5 mg/kg) injections for 30 days. During this period, half of the rats were treated with saline while the other half were given RVT (10 mg/kg/day). At the end of this period, blood pressure and echocardiographic measurements were recorded and the rats were decapitated to obtain plasma and cardiac tissue samples. Lactate dehydrogenase (LDH), creatine kinase (CK), lysosomal enzymes (β-D-glucuronidase, β-galactosidase, β-D-N-acetyl-glucosaminidase, acid phosphatase and cathepsin-D) were studied in plasma samples, while malondialdehyde (MDA), ATP and glutathione (GSH) levels and Na+/K+ ATPase and myeloperoxidase (MPO) activities were determined in the cardiac tissue samples. Caveolin-3 levels in cardiac tissues were evaluated using both histological and Western blot analyses.
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