Background
CYP3A5 has been reported to be an important genetic contributor to inter-individual and interracial differences in CYP3A-dependent drug clearance and response. The major determinant for this variation in expression was a single-nucleotide polymorphism in intron 3 at position 6986, which in the g.6986G allele (CYP3A5⁎3) led to alternative splicing of CYP3A5 transcripts and absence of CYP3A5 protein; the g.6986A allele (CYP3A5⁎1) correlated with high expression.
Materials and methods
Sixty unrelated Uzbek men with arterial hypertension I–II grade (WHO, 2003) with a mean age of 46.6±9.4 were included into the study, after receiving an informed consent. Genomic DNA was extracted from the whole blood using the Diatom DNA Prep 200 kit (Isogen Lab, Ltd., Moscow, Russia). PCR-RFLP-based analysis was performed using the CYP3A5-A6986G PCR kit (GenTest, Moscow, Russia). Statistical analysis was performed using Office Excel 2007 (Microsoft Corp., USA) and STATISTICA version 6.0 (StatSoft, Inc., USA). Student’s t test and a nonparametric chi-square ( χ 2 ) test were performed. Allele and genotype frequencies were calculated by direct counting, and Hardy–Weinberg equilibrium was evaluated by an exact test.
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