Beta-blocker use improves left ventricular ejection fraction (LVEF) in patients with heart failure. A similar effect of beta-blockers on right ventricular function has been proposed, although the effect of Bisoprolol on right ventricular function has not been assessed. This study investigated the short-term effect of Bisoprolol on right ventricular function in chronic heart failure patients. A cohort of 60 heart failure patients who were not taking beta-blockers at baseline was studied prospectively. Right ventricular ejection fraction (RVEF) and LVEF were measured at both baseline and 6 months by echocardiography. Various parameters of the right ventricular function were measured: Simpson RVEF, surface shortening fraction, right ventricular outflow tract (RVOT %), TAPSE (mm), S’ wave with tissue doppler (S’ dti cm/s), and the Tei index. The threshold of significativity was fixed at 5%. Bisoprolol was up titred during six months by a preestablished protocol to a target dose of 10 mg/Od. Mean age was 65.7 ± 16.3 years. Baseline RVEF was 25.6 ± 5.2% and baseline LVEF was 20.8 ± 6.4%. Mean Bisoprolol dose reached was 25 ± 12.5 mg daily. At 12 months, RVEF was significantly increased by 7.5% (95% confidence interval, 3.9–10.2; P = 0.0001) and LVEF also increased significantly by 7.5% (95% confidence interval, 4.0–11.9%; P = 0.0003). All the parameters of the right ventricular function were significantly improved: TAPSE (15.5 vs 12.7; P = 0.078), Doppler S’dti cm/s (10.7 vs 8.2; P = 0.002), Tei index (54.10 vs 81.45; P = 0.0008, RVOT % (27.1 vs 19.3; P = 0.036), dp/dt RV (721 vs 505; P = 0.05). The efficacy and good tolerance of bisoprolol is demonstrated in this study on chronic heart failure with right ventricular dysfunction when administered in a precise pattern.