In the present study we have analyzed if the immune response to influenza vaccine depends on the concentrations of soluble TRL-2, TRL-4, and TRL-7, and if it has any relation to the level of cytokines represented by IL-10, IL-6, IL-1β, and TNF-α. We chose to investigate the relationship between the immune response, Toll-like receptors, and cytokines in the hemodialyzed patients on the premise that immunogenicity and cytokine expression are hampered in chronic kidney disease, which might help bring out the relevant relations to the factors above outlined while comparing with healthy subjects. In addition, there is a previous study pointing to the influence of TLR on the immune response in patients with chronic kidney disease on hemodialysis (Kazimierczak et al. 2007). In contrast to that study, however, the present findings were that there was no correlation between vaccination and concentration of soluble forms of Toll-like receptors type 2, 4, and 7. Vaccination did not influence the concentrations of TLRs or cytokines in either hemodialyzed patients with compromised immunogenicity or healthy subjects. Moreover, both groups of subjects failed to show any immunogenic-responsive differences in TLRs and cytokines, despite evidently higher levels of these biomarkers in hemodialyzed patients. Understandably, increased pro-inflammatory cytokines in patients on hemodialysis point to enhanced inflammatory state in these patients.